Coding
LaG17

Part:BBa_K2549004:Design

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-01)


anti-GFP (LaG17)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 34


Design Notes

LaG17[1] is served as the extracellular domain of our SynNotch receptor, thus accomplishing contact-dependent signal input against GFP. This nanobody recognizes our surEGFP (Part:BBa_K2446051) expressing cells.

Source

From IDT (gBlock), codon optimized for human

References

  1. ↑ A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60 PMID: 25362362; DOI: 10.1038/nmeth.3170